- Pulse intensity decreases drastically at XTD2 across the train

- the visualisation of our beam therfore needs to be compensated adjusting transmission

- beam shape and uniformity is different than previous days and it is unclear how much of this is related to real changes in the beam or an effect of the slope in the train. We are therefore spending more time than usual to investigate and align becuse we cannot correct the slope.

>50 pulses was requested for alignment explicitly as this is needed to setup the instrument for measurment.

19 Feb 2023, 08:08 Hand over from night shift

Added polarizer in the PAM beam in the optics hutch, reequilibrated reference arm of PAM

19 Feb 2023, 08:09 Darks taken for JF 1 and 2

19 Feb 2023, 08:28 Check sample alignment, correct for shot samples

19 Feb 2023, 08:33 Beam was off on Zyla, M3 feedback had to be restarted this morning. May this changed the setpoint. New setpoint is now at 342.4

19 Feb 2023, 09:09 Aligned edge of Cu foil on Zyla, then moved ILM to get a sharp image of the edge, then focus RELAX beam to this position

19 Feb 2023, 09:11 OAPX motor is in permanent error state

19 Feb 2023, 09:17 Focal spot does not look nice,

19 Feb 2023, 09:40 Start shooting foils

19 Feb 2023, 09:51 Cu foil  w Al B3-A3 , delay RELAX 100 fs before x-rays, run 257, seems we destroyed foils next to it

19 Feb 2023, 10:16 DOC called, OAPX is working again, reason unknown, seems to be an issue with the encoder. Needs checking the encoder on site.

19 Feb 2023, 11:09 Finished aligning samples, preparing for shot

19 Feb 2023, 11:15 Cu foil  w Al B3-A1, delay RELAX 200 fs before x-rays, run 260

19 Feb 2023, 11:23 Cu foil B3-B2, delay RELAX 200 fs before x-rays, run 262, sample next to it was damaged

19 Feb 2023, 11:39 Cu foil B3-B3, delay RELAX 200 fs before x-rays, run 264

19 Feb 2023, 11:46 Aligning grids

19 Feb 2023, 12:07 OAPX again in error state, used this time AY rotation instead, did not really change the focal spot. Add this motors to the switcht off

19 Feb 2023, 12:14 Cu mesh 2000 C3B3, delay RELAX 200 fs before x-rays, run 266

19 Feb 2023, 12:21 Need much longer delays, will go to RF locking

19 Feb 2023, 12:43 Phase shifter device was stuck in moving state, restarted

19 Feb 2023, 12:45 Cu mesh1000 C3B1, delay RELAX 2000 ps before x-rays, run 268

19 Feb 2023, 13:00 Cu mesh 2000 C3B2, delay RELAX 500ps before x-rays, run 270

27019 Feb 2023, 13:07 Seems that RELAX does not hit exactly in the field of view, change to configuration CRL4B

19 Feb 2023, 13:19 Cu mesh HEX 1000 C3A1, delay RELAX 500ps before x-rays, run 272

19 Feb 2023, 13:22 Shot is fine, stay with CRL setting, realign samples

19 Feb 2023, 13:50 DOC called, some motor flags have been changed for OAP_X. hopefully ito works until tomorrow

19 Feb 2023, 13:54 Cu mesh2000 C2A5, delay RELAX 100ps before x-rays, run 274

19 Feb 2023, 14:00 Cu mesh2000 C2A3, delay RELAX 20 ps before x-rays, run 277

19 Feb 2023, 14:06 Cu mesh2000 C2A2, delay RELAX 20 ps before x-rays, run 280

19 Feb 2023, 14:13 Cu mesh2000 C2A1, delay RELAX 500 ps before x-rays, run 284

19 Feb 2023, 14:17 Cu mesh2000 C2B1, delay RELAX 1000 ps before x-rays, run 287

19 Feb 2023, 14:23 Cu mesh2000 C2B2, delay RELAX 1000 ps before x-rays, run 290 PPU was open before shot and closed during shot

19 Feb 2023, 14:27 Cu mesh2000 C2B3, delay RELAX 1000 ps before x-rays, run 291

19 Feb 2023, 14:31 Cu mesh2000 C2B4, delay RELAX 80 ps before x-rays, run 295

19 Feb 2023, 14:35 Cu mesh2000 C2B5, delay RELAX 666 ps before x-rays, run 298

19 Feb 2023, 14:38  Moved Siemens star in (pos 25), run 300, is out of range, tried then pos 2, visible in live view, run 301

• Improved the alignment procedure:
  • Using the pin to make sure of the spatial overlap
  • SAXS signal is used to make sure the pin is well centered on the X-ray beam path
  • YAG is only used in combination with ILM to fix with ~5 um precision the height of the beam (moving CRLs on laser focus) and the temporal overlap.
• Recording of the different laser foc planned to be used during the experiment: no aperture, 40 mm, 25 mm, 15 mm diameter

Note:

• Jet failed a couple of time
• We lost the Sync system for 2 hours, got it back again for 30 min, then lost it again

• Data taken from 11 to cryo failure
• Beam conditions stable
• Timing scan for different energy
• Operation stopped after cryo failure
• Cryo jet was heated up then
• Improvements of optical diagnostics (far field monitor)

Attch 1: We were having trouble focusing at IRU chamber. After multiple attempts the best we could achieve was 2.9-3.1 um beam.

Attch 2: We then wanted to test if this was caused by the SASE 3 background, after them changing from >350 pulses to 1, we saw an instant improvement of ~0.5um down to 2.3 um beamsize.

Attch 3: After optimizing without SASE 3 background, we got down to 1.9 um (1 um less then with SASE 3).

Attch 4: Finally, after SASE 3 changing back to their <350 pulses the beam now changed from 1.9 to 3.9 um! (instead of the previous 3.1um)

This because the SASE1+SASE3 focus =/= SASE1 focus....

We did not have much time to tune the beam without SASE 3 background, we should think about managing this tomorrow during alignment, and also for data collection...

Staff: DM, CK, KK

X-ray delivery

• 7.8keV, 2.4mj, stable delivery, homogeneos intra train energy most of the time.
• SASE 3 background

Optical laser delivery

• no laser used

Achievements/Observations

• Aligned until IRU, until before full beam test

Issues

• Valve after AGIPD was disconnected elog:12718
• SASE 3 background affecting beam focus at IRU elog 12722
• AGIPD scattering, could not solve fully with slits, to be improved tomorrow

Instrument status

• AGIPD HV off.
• AGIPD gate valve closed and pinned.
• Attenuators set to thickest diamond.
• Vacuum valves closed.

To do for next shift

• Continue with alignment, then experiment.
• Check slits or other solution for AGIPD scattering
• Confirm the beam focus impact from SASE 3 background, after we moved NKB coating (verify if it remains)

Staff: DM, CK, KK, JB, SR (SEC), LW (Uppsala university), MM (GU)

X-ray delivery

• 7.8keV, 2.4mj, stable delivery, homogeneos intra train energy most of the time.

Optical laser delivery

• ns laser for particle imaging

Achievements/Observations

• AGIPD BG optimized by opening Q2 & Q4 quadrants a few pixels and adding Pb shield on IRU_SLIT4
• NKB focused optimized
• Data (75 nm Ag cube) and gas BG (no sample) measured with two different gases (N2 and He)

Issues

• AGIPD BG near the center (1-2 pixels) were quite strong even with tight sliting, but issue solved by opening two quadrants (Q2 & Q4) and moving CSS about 1-2 pixels (elog:12731)
• Found additional AGIPD BG in Q2M1 module (mid-Q range), but issue solved by adding Pd shield on IRU_SLIT4 (elog:12734)

Instrument status

• AGIPD HV off.
• AGIPD gate valve closed and pinned.
• Attenuators set to thickest diamond.
• Vacuum valves closed.

To do for next shift

• Check focus and slit position and continue the program (data measurement).

SPB_IRU_DPA/MOTOR issue

X stage looks okay, but Y, AX, and AY values have been changed by 2-3mm. (see attachment 1 and 2)

SPB_IRU_SLIT-1/MOTOR issue

All 4 blades (top, bottom, left, and right) have been changed by 0.1-0.5mm. (see attachment 3)

SPB_IRU_SIDEMIC/MOTOR and SPB_IRU_INLINEMIC/MOTOR issue

Not fully checked, but Y & Z motors of SideMic and AX & AY motors of InlineMic have been changed by ~1mm and ~0.4 karabo unit. (see attachment 4 and 5)

Shift summary 15.2.2023 (Wednesday)

X-ray delivery

• 9.3 keV, 1-200 pulses, 1.0 mJ, pulse energy fluctuation due to SA3 tunning & special operation mode (SCS)

Optical laser delivery

• No Laser

Achievements/Observations

• LPM and Calorimeter commissioning (efficiency of MKB system and CRL lens stacks checked)
• Recorded Micron Ptychography w/ Zyla Camera
• Install EHC Shimadzu
• Both Shimadzus train synchronised and recording properly to DAQ

Issues

• 10-15% pulse energy fluctuation due to SA3
• Zyla Failure
• Poor scattering efficiency on Zyla

X-ray delivery

• 9.3 keV, 1-120 pulses

Optical laser delivery

• No Optical Laser

Achievements/Observations

• EHC Shimadzu timing and focusing
• Two-Plane Measurements Tested
• SA1, NKB and EHC Shimadzu flatfields recorded
• Coherence measurement with EHC Shimadzu

Issues

• Bassler cameras misbehaving

SPB/SFX User Proposal 3333 Shift 2 Summary

X-ray delivery

• 9.3 keV, 1.7 mJ, 202 pulses @ 564 kHz

• 2 pulse trial @ 2.8 kHz (2.2MHz / 800). Doubled data rate for high viscocity jet. Seemed to work quite well although not expected to be a regular use mode!

Optical laser delivery

• Only 819 nm ns laser for the jet visualization

Achievements/Observations

• Data calibration issues solved (thanks to DRC / ITDM experts)

• Silicon wafer post sample attenuator was dismounted. elog: 45

• High viscosity jet delivery @ 10 Hz and 2.8 kHz (2 pulses / train @ max spacing)

• Photosystem-II (@ 41 mm AGIPD Z motor value, adaptive gain) elog: 85,86, various sample preparations

Issues

• ONDA restarts required

Instrument status

• Sample chamber and AGIPD turbos OFF, rough pumping ON

• shutters etc. closed

To do for next shift

• Catcher and shroud (inc windows) could do with a clean before any alignment (PEG delivery)

• Proceed with PCS users

X-ray delivery

• 12.42 keV, 0.8 mJ/pulse, stable delivery

Optical laser delivery

• none

Achievements/Observations

• BG optimized (1-2 photons)
• Realignment of offset mirrors (M1 & M2), cube, straw, sidemic
• Jungfrau4M timing checked and optimized for single memory cell operation
• About 2 hours data collection

Issues

• Low pulse energy (Tomorrow, BKR and we should optimize the pulse energy)
• Extremely slow data calibration (it took more than 1 hour for 10 minutes run)

X-ray delivery

• 12.42 keV, 0.65 mJ/pulse, low pulse energy but mostly stable delivery

Optical laser delivery

• none

Achievements/Observations

• Unit photon flux increased by bending M2 bender more although incident pulse energy was about 0.65 mJ/pulse (10-20% lower then yesterday)
• Long data collection (4.5 hours) although we had rather low hit rate
• SideMic position and focus optimized in the end of the shift

Issues

• Even lower pulse energy (M2 bender helped a bit), any improvement will be helpful for Sun. night shift

X-ray delivery

• 12.42 keV, stable delivery with slightly less than 1.0 mJ/pulse (~0.95 mJ/pulse)

Optical laser delivery

• none

Achievements/Observations

• BKR semi-optimized photon flux by ~20% more then yesterday)
• Long data collection (5 hours) although we still had rather low hit rate with ligand (hit rate was higher without ligand)

Issues

• Beam pointing issue (only relavent to SPB_EHC_CRL) after BKR semi-optimize photon flux
• Rather low hit rate due to flux, crystal size, ligand, etc.

Vacuum issue at XTD2 tunnel which might be triggered by SA1_XTD2_ATT Arm5 (1.2mm thick CVD diamond)

It happened once again at around 3pm.

X-ray delivery

• 7.8 keV, up to 260 pulses at 1.1MHz, >4 mJ

Optical laser delivery

• PP laser was down since 00:48.

Achievements/Observations

• NKB focus optimized and beam cleaning partially done
• Damaged IRU slit-4 has been rearranged and replaced
• Stable AGIPD operation

Issues

• Beam on AGPID seems still too big and beam cleaning did not really work (we may need to close PSLITs during the next shift)
• IRU_SLITs are too close to the X-ray beam and one blade in IRU_SLIT-4 was damaged by X-ray
• ~10 pixels in Q2M1 overexposed
• SA1 PPL Amphos down since 00:48

X-ray delivery

• 7.8 keV, up to 260 pulses at 1.1MHz, ~3 mJ

Optical laser delivery

• PP laser, stable delivery

Achievements/Observations

• NKB focus optimized with reduced PSLIT (both MKB and EHU) opening
• Found the orgin of the BG (mostly from AGIPD gun shield)
• Stable AGIPD operation

Issues

• Beam slitting with PSLITs & IRU_SLITs does not help in reducing the BG from the AGIPD Gun-shield
• Placing additional Pb shield on the injector tip does not help (BG source is in between injector and AGIPD)

SA3 background is very strong and it affects our NKB (nano KB, Ru coating) focus.

Attachment 1: optimized NKB focus with 1 pulse @SA3

Attachment 2: optimized NKB focus + SA3 BG with 375 pulses @SA3

Attachment 3: optimized NKB focus with 375 pulses @SA3

Attachment 4: optimized NKB focus with 375 pulses @SA3, but after changing number of pulses to 1 @SA3

Issue solved (elog:410) after changing NKB mirror coating to B4C.