The status of the XTD2 attenuators has been checked during the experiment last week and many were found to cause disturbances in the wavefront ranging from mild to severe.

Attachment 1: No attenuator
Attachment 2: 75 um CVD - Fringes visible in beam
Attachment 3: 150 um CVD - Strong fringes in beam
Attachment 4: 300 um CVD - Looks more or less ok
Attachment 5: 600 um CVD - Weak distortion in the beam profile
Attachment 6: 1200 um CVD - Ok
Attachment 7: 2400 um CVD - Ok
Attachment 8: 500 um Si - Not ok

X-ray delivery

• 10 keV, up to 160 pulses at 1.1MHz, 1.8 mJ

Optical laser delivery

• NA

Achievements/Observations

• Instrument aligned up to IRDa at 10 keV.
• Focus optimised.
• JUNGFRAU timed.

Issues

• We could only start using the beam at 1 am.
• More mirror movement needed to get the beam down the instrument than expected.
• Several AGIPD power cycling needed due to issues with MFPGA2

X-ray delivery

• 10 keV, up to 128 pulses with custom bunch pattern, 1.6-2.0 mJ

Optical laser delivery

• NA

Achievements/Observations

• Collected first SFX data, but background on JUNGFRAU is rather high and needs further optimisation.

Issues

• AGIPD having frequent problems with both MFPGAs (once even both at the same time). Frequent restarts were needed.
• Horizontal drift of the beam pointing.

The number of bunches during the last night shift kept being changed down to 24 by the device SA1_XTD2_WATCHDOG/MDL/ATT1_MONITOR020 acting on SA1_XTD2_BUNCHPATTERN/MDL/CONFIGURATOR. This was not the case during the previous shifts, even though the pulse energy was comparable. Looking at the history of SA1_XTD2_WATCHDOG/MDL/ATT1_MONITOR020, it looks like this device was started at 16:26:25 on Friday, 1st March.

• 10 keV beam
• Detector: JUNGFRAU in the burst mode (16 memory cells)
• Achievements:
  • multiple sfx data sets (lysozyme) with 4 and 8 pulses (at 1.1MHz) per exposure taken

We checked the XTD2 attenuators following the intervention from XRO (compar ewith elog:37, before the intervention).

The wavefront shows similar distortions with the 75 and 150 um as earlier, which is not too surprising since the cracks are vertical.

Attachments 1-5: Beam on SPB_XTD9_SCR with XTD2 attenuator CVDs

Attachments 6-7: Beam on FEL imager with XTD2 75 and 150 um CVDs

X-ray delivery

• 6 keV, 2.6 mJ/pulse, stable delivery

Optical laser delivery

• 800nm PP laser, stable delivery

Achievements/Observations

• NKB focus optimized and PSLITS optimized
• Beam going through IBS camera
• PP laser rough timing checked w.r.t. X-ray
• Catcher and injector motors (X, Z) are aligned well with about 1.5mm offset in X

Issues

• Manifold-MDL issue (reported in Issues tap)
• SPB_IRU_LIQUIDJET/CAM/1 (IRU nozzle insertion camera) is not working. Power cycle required.
• SPB_EHU_Valve closed 2-3 times due to vacuum interlock
• Space (KF50 pipes) in the super-structure was too long for the user injector (nozzle rod). After taking off small spacer, nozzle is very close to the injector valve during insertion. Please check it out whether we can find any better solution or not.

X-ray delivery

6 keV, 2.4 mJ/pulse, stable delivery

Optical laser delivery

800nm PP laser, stable delivery

Achievements/Observations

NKB focus cslits optimized
Some progress with AGIPD background although we still need to improve
Catcher and injector motors (X, Z) are aligned well; with defroster, had under 5mm offset in X
Managed to get a jet and overlap with X-rays.

Issues

Beckhoff flowmeters were disconnected and we were not sure which ports to connect, maybe spent 45min to connect to the right ports and restart device.
Helium flow meter was on but not updating, had to restart it too.
Marco noted actual measured flow at beckhoff VS HPLC set flow was perhaps too low, not sure
Vacuum with injector improved, issue was related to nozzle preparation, however even after this fix the chamber pressure was just over the AGIPD interlock. After tuning gas/liquid flow managed to go under it, but possibly this can also be improved.
Often number of pulses was being changed without us requesting it.
One AGIPD module stopped working after moving quadrant.
DAQ data aggregator crashed a few times, Steffan Hauf will look into this issue after we finished the shift.
Could not move one of the AGIPD quadrant (Q4) due to interlock
Background in Q1 and Q3 needs to be optimized, and/or quadrants needs to be moved.

There are now two watchdogs for the XTD2 attenuator running at the same time

• SA1_XTD2_WATCHDOG/MDL/ATT1_MONITOR020
• SA1_XTD2_OPTICS_PROTECTION/MDL/ATT1

that are configured differently, which results in different pulse limits (see attachment).

**X-ray delivery**

- 6 keV, 3.2 mJ/pulse, stable delivery

**Optical laser delivery**

- 800nm PP laser for jet illumination, stable delivery

**Achievements/Observations**

- NKB focus checked and IUT_Slits optimized
- AGIPD quadrants position finalized
- Measured a lot of water BG runs and few runs with Au nanoparticles (sphere with ~50 nm in diameter)

**Issues**

- SideMic data not saved properly (empty array).
- Found relatively high BG when nozzle is IN since the distance in between nozzle tip & the interaction region is ~100um (even 80 um in some cases).
- DA issue (DAQ was stuck in changing) although all the DA looked healthy. DOC fixed the issue (temporarly) together with Egor, Florian, and Steffen.
- Runs callibration were not working

X-ray delivery
- 9.3 keV, >3 mJ/pulse, stable delivery 

Optical laser delivery
- 800nm PP laser for jet illumination, stable delivery

Achievements/Observations
- Optimised hexapod position for SFX
- Collected granulovirus data for geometry calibration
- Started user sample delivery but clogged the nozzle a few times

X-ray delivery
- 9.3 keV, >3 mJ/pulse, beam down around 30min

Optical laser delivery
- 800nm PP laser for jet illumination, stable delivery

Achievements/Observations
- Collected a lot of data (from midnight to 6am) with different sample concentrations and mixing delays with the users

X-ray delivery

• 12.72 keV, 10 Hz, NOT stable delivery, pointing drastically changed in the afternoon, presumably due to the train-on-demand operation at SASE2

Optical laser delivery

• 487 nm ns laser (not used)
• 800 nm PP laser for sample illumination

Achievements/Observations

• Beam is aligned for IRDa
• lysosyme date was collected for JF4M geometry

Issues

• X-ray pointing changed in the afternoon, we have to figure out why and whether we can avoid losing trajectory
• Beam is currently clipped and EHC_SCR, @Romain Letrun , @Adam Round please take a look tomorrow
• Detector background was not optimal, might require more work tomorrow
• Roadrunner RX motor did not move, in Karabo, was giving error messages @Adam Round please take a look, maybe resetting the roadrunner outside of Karabo can help
• AGIPD MFPGA2 issue (keep acquiring) which was solved by power cycle

Pulse energy fluctuation observed after full train operation in SA2 starts.

X-ray delivery
- 12.72 keV, 10Hz, ~1.4 mJ/pulse, stable delivery 

Optical laser delivery
- 487 nm ns laser for pump-probe
- 800 nm PP laser for jet illumination, stable delivery

Achievements/Observations
- AGIPD BG optimized (DiffPump realigned)
- IRDA focus optimized (EHC_CRL arm10 (1mm pinhole) inserted)
- Completed dark state measurement of the main sample (more than 4 hours of measurement time)
- Collected PP (pump-probe) dataset with 5 us time delay (about 3 hours of measurement time; Jet was quite stable during the 3 hrs measurement)

Issues
- AGIPD module Q3M2 was not healthy. After another power cycle, it recovered.
- X-ray pulse energy was relatively low in the beginning of the shift. It was partially recovered after running the launch optimization by BKR.

DOOCS panels are again incorrectly reporting the FXE shutter as opened when it is in fact closed. This had been fixed after it was last reported in February (elog:24), but reappeared now.

Continuous pointing drift in horizontal direction.

It may trigger a vacuum spike at SPB OH (MKB_PSLIT section) at 21:07 pm.

X-ray delivery

- 12.72 keV, 10Hz, ~1.4 mJ/pulse, continuous beam drift in the horizontal direction observed

Optical laser delivery
- 487 nm ns laser for pump-probe
- 800 nm PP laser for jet illumination, stable delivery

Achievements/Observations
- Collected PP (pump-probe) dataset with 5 us time delay more than 12 hours
- Collected 3 runs (5 minutes run) of dark state in the end of the shift
- Jet was quite stable most of the time

Issues
- Continuous beam pointing drift in the horizontal direction observed
- Vacuum spike at MKB_PSLIT section trip the beam shortly which might be related to the beam pointing drift

X-ray delivery

• 12.72 keV, 10Hz, ~1.4 mJ/pulse, some beam drift in the horizontal direction observed (probably mirror related)

Optical laser delivery

• 487 nm ns laser for pump-probe
• 800 nm PP laser for jet illumination, stable delivery

Achievements/Observations

• Collected PP (pump-probe) dataset with 5 us time delay more than 12 hours
• Jet and data collection was stable most of the time