X-ray delivery:

10 keV, ~ 900 uJ, 128 pulses, tuning was needed for few hours

Achievements / Observations:

Alignment of crystals

Issues:

X-ray beam intensity was weak at 12.7 keV (100 uJ), so we decided to use 10 keV

X-ray delivery:

10 keV, ~ 850 uJ, 128 pulses, tuning was needed for 1 hour 

Optical Laser:

No PP-laser for this week

Achievements / Observations:

Finalized alignment of crystals

Issues:

No major issue today.

Staff: PV, TS, JK + external collaborators 

X-ray delivery:

10 keV, ~ 800 uJ, 128 pulses, tuning was needed for 10 min. 

Optical Laser:

No PP-laser for this week

Achievements / Observations:

Finalized alignment of X-ray setup

Issues:

No major issue today.

X-ray delivery

• 9.3 keV, 1800 uJ pulse energy

• 1 - 202 pulses at 564 kHz

• Poor beam stability overall. Lots of intensity fluctuations and rather long time to recover after change of number of pulses in any beamline.

Optical laser delivery

• 800 nm, 113 pulses, 564 kHz stable delivery

• Illumination laser aligned

Achievements/Observations

• Aligned instrument

• Prepared illumination laser

• Shot PCS sample for a few minutes

Issues

• PCS sample clogged (at filter not nozzle) after a few crystal shots

• A few clogs with nozzle with lyzo, when X-ray were on, even far from the nozzle somehow the splash from the explosion grew on the tip

X-ray delivery

• 8.3 / 9.3 keV, 1400 uJ pulse energy, 1 hour down time

Optical laser delivery

• NS illumination
• 355 nm pump laser

Achievements/Observations

• PCS finished
• Preparation for the next experiment; mainly 355 nm.

**X-ray delivery**
- 8.3 , 1600 uJ pulse energy

**Optical laser delivery**
- NS illumination
- 355 nm pump laser

**Achievements/Observations**
- Measured/tested various samples and different pump laser delays and illumination delays

**Issues**
- There was some sample that caused clogging in the morning

X-ray delivery

• 8.0 keV, 1600 uJ pulse energy

Optical laser delivery

• NS illumination
• 355 nm pump laser

Achievements/Observations

• Once again measured a lot of runs worth of data today

Issues

• No issues

X-ray delivery

• 8.0 keV, 1300 uJ pulse energy by the end, it lowered during the day

Optical laser delivery

• NS illumination
• 355 nm pump laser

Achievements/Observations

• Once again measured a lot of runs worth of data today

Issues

• No major issue today

X-ray delivery

• 8.0 keV, 1300-1600 uJ pulse energy by the end

Optical laser delivery

• NS illumination
• 355 nm pump laser

Achievements/Observations

• Once again measured a lot of runs worth of data today

Issues

• No major issue today

Shift summary in-house beamtime night 1

X-ray delivery

• 9.3 keV, 1.7-2.2 mJ pulse energy, up to 202 pulses at 0.5MHz

Optical laser delivery

• NS illumination
• PP laser not available as we could not change master experiment

Achievements/Observations

• Collected SFX lysozyme data at two different detector distances
• Collected SFX metmyoglobin as positive control

Issues

• None

Staff: @**Katerina Doerner**  @**Elizabeth Galtry** @**Raphael de Wijn** @**Mohammad Vakili** @**Chan Kim** @**Konstantin Kharitonov** 

**X-ray delivery**
- 9.3 keV, 1400-1600 uJ pulse energy

**Optical laser delivery**
- No

**Achievements/Observations**
-  Found working nozzle, started injection, observed droplets, performed two parameter scans for HV 
- We could not reach the  stable droplet  frequency around 10 Hz  and were ~  fluctuating 9-17 Hz
- We  prepaired  3 more capillaries  with powder for distance calibration and left them in D12

**Issues**
- Some nozzles were leaking

**Instrument status**
- AGIPD HV off.
- AGIPD gate valve closed and pinned.
- Attenuators set to thickest diamond.
- Vacuum valves closed.
- AGIPD breaks engadged
- ns Lasers off
- HIREX  is handled by FXE, but maybe better to check in the morning

- AGIPD IS REMOVED FROM DAQ  TO MEASURE DROPLET TRAINS DON'T FORGET TO PUT IT BACK

Staff: @**Katerina Doerner**  @**Elizabeth Galtry** @**Raphael de Wijn** @**Mohammad Vakili** @**Chan Kim** @**Diogo Melo** 

**X-ray delivery**
- 9.34 keV, 1400-1600 uJ pulse energy

**Optical laser delivery**
- illumination laser

**Achievements/Observations**
-  Collected some NQO1 data
- Had some hours to play around with HV with the old device. We observed a few things:
     - Without HV we could get close to 10 hz, but with jitter of +/-3hz
     - With HV at 1.2V, we clearly saw a strong effect on the droplet hz, could go from 10 Hz to 20 Hz, but the problem of the jitter remains, this looks like the main issue have the droplets frequency under control
     - We observed that with water the diode signal was one single peak, whereas with sample there were a few peaks for the whole droplet width, we tested and it indeed seems that the crystals in the droplets either refracted away or absorbed a portion of the beam to yield that diode laser peak profile...

**X-ray delivery**
- 9.34 keV, 1400-1600 uJ pulse energy

**Optical laser delivery**
- illumination laser

**Achievements/Observations**
-  Got crystal hits with a device, bypassing the valve, and most importantly the filter. We checked a drop before and after filter and there were no crystals after the filter.
- Managed to control the droplet formation for some periods - but with crystals being blocked by the filter. Did not manage to synchronize the droplets to the X-ray though.

Staff: @**Katerina Doerner**  @**Elizabeth Galtry** @**Raphael de Wijn** @**Mohammad Vakili** @**Chan Kim** @**Diogo Melo** @**Konstantin Kharitonov** 

**X-ray delivery**
- 9.34 keV, 1200-1400 uJ pulse energy

**Optical laser delivery**
- illumination laser

**Achievements/Observations**
-  Managed to synchronize droplets for some runs, resulting in high hit, averaging rate ~ 8-10% and spiking up to 20%.

**Issues**
-  Synchronization and stable delivery  are challenging 
- Beam was down several times

**Instrument status**
- AGIPD HV off.
- AGIPD gate valve closed and pinned.
- Attenuators set to thickest diamond.
- Vacuum valves closed.
- AGIPD breaks engadged
- ns Lasers off
- HIREX  is handled by FXE, but maybe better to check in the morning
- PAM interlock on and shutter open
- Left the gas at 5 for 0 flow (and no noise) from GP13 flowmeter D, during the night it was at 0, and in the morning it was filled with air... Tonight we leave it this way and in the morning let us know if it worked or not.

**X-ray delivery**
- 9.34 keV, 1200-1400 uJ pulse energy

**Optical laser delivery**
- illumination laser

**Achievements/Observations**
-  Measuired multiple runs data with synchronization and high hit rate
- We have at least two devices tested and ready for tomorrow
 

X-ray delivery

• 9.3 keV, 2000 uJ pulse energy. Beam was unstable at the beginning.

Optical laser delivery

• ns laser for particle illumination inside the chamber,
• another ns laser to visualize the tip of capillary on top of the chamber

Achievements/Observations

• Measured GV for ~ 2 hours

Issues

• PSI from the first reservoir was leaking
• Concentration of PSI seems low (judged by seeing camera for liquid jet delivery).
• We could not see any diffraction from PSI by both AGIPD preview and OnDA (same setting with GV used)

Instrument status

• AGIPD HV off.
• AGIPD gate valve closed and pinned.
• Attenuators set to thickest diamond.
• Vacuum valves closed.
• AGIPD breaks enguaged
• ns Lasers off

X-ray delivery

• 9.3 keV, 2000 uJ at the beginning, 1600 uJ after 2AM. There was downtime around 15 minutes due to gun issue.
• FXE used the beam until 23:30.

Optical laser delivery

• ns laser for particle illumination inside the chamber,
• another ns laser to visualize the tip of capillary on top of the chamber

Achievements/Observations

• Measured PSI
• Hit rate is extremely low. We could see some peaks from AGIPD preview, but it was difficult to see it from the hit finder.

Issues

• Sample capillary line was clogged twice.
• From the images of capillary tip and particle scattering (rayleigh / mie scattering), it seems it works fine. But hit rate is too low.

Instrument status

• AGIPD HV off.
• AGIPD gate valve closed and pinned.
• Attenuators set to thickest diamond.
• Vacuum valves closed.
• AGIPD breaks enguaged
• ns Lasers off
• HIREX is out

X-ray delivery

• 9.3 keV, 1400 uJ photon flux

Optical laser delivery

• ns laser for particle illumination inside the chamber
• another ns laser to visualize the tip of capillary on top of the chamber

Achievements/Observations

• Measured GV for ~2 hours, hit rate is ~0.0003 (from Karabo TST/SFX/MONITOR/sfx, 1 = 100 %)
• Measured PSI with different condition as below :
  1) without dryer : as we observed sample deposited on the tube connected to the inlet of dryer yesterday. Black tube is coated to avoid any deposition of particle on the wall)
  2) without both lasers : to avoid any heat effect if laser produces heat
  3) without EtOH : EtOH can disolve PSI crystals

Issues

• PSI sample is not delivered well to the interaction region.
• When adjust the skimmer at first, I did not run liquid flow. After the skimmer distance is adjusted to the reference pressure on Pressure at injector position, we started run sample and it increased pressures not only on pressure next to the skimmer and injector inlet, but also the pressure of IRU chamber. So it triggered the valve at upstream of IRU chamber (SPB_IRU_VAC/VALVE/VALVE_INLINE_1) to close. It is solved by adjusting skimmer gap again while the liquid flow is running.

Instrument status

• AGIPD HV off.
• AGIPD gate valve closed and pinned.
• Attenuators set to thickest diamond.
• Vacuum valves closed.
• AGIPD breaks enguaged
• ns Lasers off
• HIREX is out