X-ray delivery

• 9.3 keV, 2000 uJ pulse energy. Beam was unstable at the beginning.

Optical laser delivery

• ns laser for particle illumination inside the chamber,
• another ns laser to visualize the tip of capillary on top of the chamber

Achievements/Observations

• Measured GV for ~ 2 hours

Issues

• PSI from the first reservoir was leaking
• Concentration of PSI seems low (judged by seeing camera for liquid jet delivery).
• We could not see any diffraction from PSI by both AGIPD preview and OnDA (same setting with GV used)

Instrument status

• AGIPD HV off.
• AGIPD gate valve closed and pinned.
• Attenuators set to thickest diamond.
• Vacuum valves closed.
• AGIPD breaks enguaged
• ns Lasers off

X-ray delivery

• 9.3 keV, 2000 uJ at the beginning, 1600 uJ after 2AM. There was downtime around 15 minutes due to gun issue.
• FXE used the beam until 23:30.

Optical laser delivery

• ns laser for particle illumination inside the chamber,
• another ns laser to visualize the tip of capillary on top of the chamber

Achievements/Observations

• Measured PSI
• Hit rate is extremely low. We could see some peaks from AGIPD preview, but it was difficult to see it from the hit finder.

Issues

• Sample capillary line was clogged twice.
• From the images of capillary tip and particle scattering (rayleigh / mie scattering), it seems it works fine. But hit rate is too low.

Instrument status

• AGIPD HV off.
• AGIPD gate valve closed and pinned.
• Attenuators set to thickest diamond.
• Vacuum valves closed.
• AGIPD breaks enguaged
• ns Lasers off
• HIREX is out

X-ray delivery

• 9.3 keV, 1400 uJ photon flux

Optical laser delivery

• ns laser for particle illumination inside the chamber
• another ns laser to visualize the tip of capillary on top of the chamber

Achievements/Observations

• Measured GV for ~2 hours, hit rate is ~0.0003 (from Karabo TST/SFX/MONITOR/sfx, 1 = 100 %)
• Measured PSI with different condition as below :
  1) without dryer : as we observed sample deposited on the tube connected to the inlet of dryer yesterday. Black tube is coated to avoid any deposition of particle on the wall)
  2) without both lasers : to avoid any heat effect if laser produces heat
  3) without EtOH : EtOH can disolve PSI crystals

Issues

• PSI sample is not delivered well to the interaction region.
• When adjust the skimmer at first, I did not run liquid flow. After the skimmer distance is adjusted to the reference pressure on Pressure at injector position, we started run sample and it increased pressures not only on pressure next to the skimmer and injector inlet, but also the pressure of IRU chamber. So it triggered the valve at upstream of IRU chamber (SPB_IRU_VAC/VALVE/VALVE_INLINE_1) to close. It is solved by adjusting skimmer gap again while the liquid flow is running.

Instrument status

• AGIPD HV off.
• AGIPD gate valve closed and pinned.
• Attenuators set to thickest diamond.
• Vacuum valves closed.
• AGIPD breaks enguaged
• ns Lasers off
• HIREX is out

X-ray delivery

• 9.3 keV, 1600-1700 uJ photon flux, beam down once due to Big Brother intervention. But the signal on HIREX was less than 10 counts.

Optical laser delivery

• ns laser for particle illumination inside the chamber
• another ns laser to visualize the tip of capillary on top of the chamber

Achievements/Observations

• Measured granulovirus

• Hit rate of defocused beam was similar or less than with focused beam, Q range is smaller than with focused beam.

• Without dryer, hit rate is more than twice.

Issues

• BIG BROTHER is intervened due to HIREX and beam was down due to that. But the count on HIREX with Al1.2 was less than 100 count.

Instrument status

• AGIPD HV off.
• AGIPD gate valve closed and pinned.
• Attenuators set to thickest diamond.
• Vacuum valves closed.
• AGIPD breaks enguaged
• ns Lasers off
• HIREX is out

SPB/SFX 7955 Day 4

X-ray delivery

• up to 202 pulses per train (~1.4 mJ pulse energy), 0.5 MHz

• stable

Optical laser delivery

• 420nm used for sample excitation. timed to 1ps with water jet interaction

• PAM set up for time points <1 ps

• From 19:15 to 21:15 (100 uJ/cm2 data point) the feedback loops were off resulting in higher intensity fluctuations, resolved at 21:52

Achievements/Observations

• x-ray beam de-focused to a few um for data taking

• PP laser aligned and timed

• PAM calibrated and used with YAG sample surviving 13% transmission

• power titration at 100ns and fluences 13 mJ/cm2, 25 mJ/cm2, 100 mJ/cm2 and 191 mJ/cm2

• delays 100 fs and 500 fs

• good peak distribution at 13% transmission (None / None / None / 3200 CVD)

• hit rate around 6%

Issues

• problems with catcher alignment after clean up

SPB/SFX 7955 Shift 1
**X-ray delivery**
* up to 202 pulses per train (~1.4 mJ pulse energy), 0.5 MHz.

**Optical laser delivery**
* 420nm used for sample excitation. timed to 1ps with water jet interaction
* difficult to find good filter combination for jet illumination laser and pump laser

**Achievements/Observations**
* 10.8 keV - 11.2 keV energy scan
* lysozyme geometry data set
* several runs for sample dark data set (potentially more needed)
* a few runs for 1ps pumped data set (definitely more needed)

**Issues**
* difficult end to shift (nozzle and illumination issues)
* hit rate rather low for timing program (leads to long processing times)

**To do for next shift**
* continue user data set including power titration
* start time delay sequence

X-ray delivery

    up to 352 pulses per train (~1.9 mJ pulse energy)@9.3 keV, 0.5 MHz ans 1.1 MHz. Beamdown for 40 min.

Optical laser delivery

    800 nm, less than 20 fs at sample position, no issue

Achievements/Observations

    X-ray beam de-focused to a few um for data taking

    PP laser aligned and timed

    1 hours data collection

Issues

    AGIPD gain map was strange, some gain changes was observed without X-ray

    Data was not migrated -> DRC was called and informed

X-ray delivery

    up to 202 pulses per train (~1.9 mJ pulse energy)@9.3 keV, 0.5 MHz

Optical laser delivery

    800 nm, less than 20 fs at sample position, no issue
    

Achievements/Observations

    X-ray beam de-focused to a few um for data taking

    PP laser aligned and timed

    runs for -1 ps - 1.5 ps

    Very stable jet

Issues
    No major issue

X-ray delivery

    up to 202 pulses per train (~1.9 mJ pulse energy)@9.3 keV, 0.5 MHz

Optical laser delivery

    800 nm, less than 20 fs at sample position, no issue

Achievements/Observations

    X-ray beam de-focused to a few um for data taking

    PP laser aligned and timed

    runs for -1 ps - 1.5 ps

    Very stable jet

Issues

    No major issue

X-ray delivery

    up to 202 pulses per train (~1.9 mJ pulse energy)@9.3 keV, 0.5 MHz

Optical laser delivery

    800 nm, less than 20 fs at sample position, no issue

Achievements/Observations

    X-ray beam de-focused to a few um for data taking

    PP laser aligned and timed

    runs for -1 ps - 1.5 ps

    Very stable jet

    PAM was installed

X-ray delivery

    up to 202/352 pulses per train (~0.8 mJ pulse energy)@13.4 keV, 0.5/1.1 MHz 

Optical laser delivery

    800 nm, less than 20 fs at sample position, no issue

Achievements/Observations

    X-ray beam de-focused to a few um for data taking

    PP laser aligned

    Fixed target measurement was finished

X-ray delivery

    up to 202/352 pulses per train (~0.8 mJ pulse energy)@13.4 keV, 0.5/1.1 MHz 

Optical laser delivery

    800 nm, less than 20 fs at sample position, no issue

Achievements/Observations

    X-ray beam de-focused to a few um for data taking

    PP laser aligned

    Data collection for some samples

Issues

    No major issue today

X-ray delivery

    up to 202/352 pulses per train (~0.8 mJ pulse energy)@13.4 keV, 0.5/1.1 MHz 

Optical laser delivery

    800 nm, less than 20 fs at sample position, no issue

Achievements/Observations

    X-ray beam de-focused to a few um for data taking

    PP laser aligned

    Data collection for some samples
 

X-ray delivery

    up to 175 pulses per train (~1 mJ pulse energy)@13.4 keV, 0.5 MHz

Optical laser delivery

    800 nm, less than 20 fs at sample position, no issue

Achievements/Observations

    X-ray beam de-focused to a few um for data taking

    PP laser aligned

    Data collection for some samples

Issues

    We could use 188 pulses, but last 13 pulses intensity was too weak, not used for measurement

X-ray delivery

    up to 188 pulses per train (~1 mJ pulse energy)@13.4 keV, 0.5 MHz

Optical laser delivery

    800 nm, less than 20 fs at sample position, no issue

Achievements/Observations

    X-ray beam de-focused to a few um for data taking

    PP laser aligned

    Data collection for some samples

X-ray delivery

    up to 188/352 pulses per train (~1 mJ pulse energy)@13.4 keV, 0.5/1.1 MHz

Optical laser delivery

    800 nm, less than 20 fs at sample position, no issue

Achievements/Observations

    X-ray beam de-focused to a few um for data taking

    PP laser aligned

    Data collection for some samples

**X-ray delivery:**
* 16 keV, ~upto 1 mJ, 160 pulses
**Optical Laser:**
* None
**Achievements / Observations:**
* Alignement
* Recorded first explosions

X-ray delivery:

20 keV, ~ 400 uJ, 128 pulses, stable delivery 

Optical Laser:

1030 nm, 850 fs, stable delivery

Achievements / Observations:

20 keV beam to sample position and cameras
Rough alignment of optical setup
 

Issues:

Optical laser from light conversion had issue with shutter control

X-ray delivery:

20 keV, ~ 400 uJ, 128 pulses, stable delivery 

Optical Laser:

1030 nm, 850 fs, stable delivery

Achievements / Observations:

Rough timing alignment of optical laser setup
Took some initial data 

Issues:

No major issue to report