SPB/SFX User shift 1 3333

X-ray delivery

• 9.3 keV, 1.7 mJ, 202 pulses @ 564 kHz

Achievements/Observations

• Silicon wafer post sample attenuator (240um, ~10% transmssion @ 9.3 keV) was mounted in-front of the AGIPD detector. elog: 3

• Beamline is aligned for the NKB trajectory and the x-ray focus size of 300 nm being achieved. elog: 4

• Copper flat field including 240 um silicon wafer attenuator. elog : 7

• Lysozyme and Photosystem-II data (@ 41 mm AGIPD Z motor value) elog: 5

• Fixed medium gain used initially, later switched to adaptive gain to allow high gain for peaks behind Si attenuator mask. Switch of both calibration pipelines..

• data at full transmission. Move to medium gain for water ring outside Si attenuator

• Several blocked nozzles, at least potentially caused by never filtered sample.

Issues

• Problems with post calibration analysis, observing 'disk quota error' - SPB/SFX RC to investigate with instrument data team and DRC

• problems with data calibration - SPB/SFX RC to investigate with instrument data team and DRC

Instrument status

• AGIPD off and warm

• AGIPD gate valve OPEN and pinned.

• Attenuators set to thickest diamond.

• Vacuum valves closed.

• He valve closed.

• Sample valves cleaned.

• ns laser off.

• Sample chamber and AGIPD turbos OFF, rough pumping ON

To do for next shift

• remove Si post-sample attenuator

• continue with user program (small crystals DFFN etc. see elog)